منابع مشابه
SNARE Zippering and Synaptic Strength
Synapses vary widely in the probability of neurotransmitter release. We tested the hypothesis that the zippered state of the trans-SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) complex determines initial release probability. We tested this hypothesis at phasic and tonic synapses which differ by 100-1000-fold in neurotransmitter release probability. We injected, p...
متن کاملSynaptotagmin 1 and Ca2+ drive trans SNARE zippering
Synaptotagmin 1 (Syt1) is a major Ca(2+)-sensor that evokes neurotransmitter release. Here we used site-specific fluorescence resonance energy transfer (FRET) assay to investigate the effects of Syt1 on SNAREpin assembly. C2AB, a soluble version of Syt1, had virtually no stimulatory effect on the rate of the FRET at N-terminus of SNARE complex both with and without Ca(2+), indicating C2AB does ...
متن کاملAlternative zippering as an on-off switch for SNARE-mediated fusion.
Membrane fusion between vesicles and target membranes involves the zippering of a four-helix bundle generated by constituent helices derived from target- and vesicle-soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). In neurons, the protein complexin clamps otherwise spontaneous fusion by SNARE proteins, allowing neurotransmitters and other mediators to be secreted...
متن کاملInterference of Zippering of Snare Complexes by Alpha-Snap Arrest Fusion of Chromaffin Granule
2562-Pos Board B254 Direct Detection of Reconstituted, Snare-Mediated Fusion Pore Dynamics Zhenyong Wu, Erdem Karatekin. Department of Cellular and Molecular Physiology, Yale University, New Haven, CT, USA. Exocytosis underlies release of neurotransmitters and hormones. Electrophysiological and electrochemical measurements from live cells have shown that the initial fusion pore is small (~1 nm ...
متن کاملα-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery.
Neuronal exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Before fusion, SNARE proteins form complexes bridging the membrane followed by assembly toward the C-terminal membrane anchors, thus initiating membrane fusion. After fusion, the SNARE complex is disassembled by the AAA-ATPase N-ethylmaleimide-sensitive factor that requires...
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ژورنال
عنوان ژورنال: Bioscience Reports
سال: 2016
ISSN: 0144-8463,1573-4935
DOI: 10.1042/bsr20160004